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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Apr 19;1863(7 Pt A):1633–1642. doi: 10.1016/j.bbamcr.2016.04.018

Figure 2.

Figure 2

Compression-induced HDAC4 nuclear import in chondrocytes. (A) Confocal microscopy showed that HDAC4 was mainly located in the nucleus of cells subjected to 3 h compression (A-k to t) when compared to unloaded cells (A-a to j). Blue indicated cell nuclei stained by Hoechst 33342, and green indicated the GFP-HDAC4 stain. (B) Percentage of green GFP-HDAC4 located in nucleus only. Approximately 300 cells from 3 independent experiments were scored. Data are expressed as means±SD (P=0.0009). (C) Cytoplasmic and nuclear lysates from the cells were separated by using the Nuclear Extract Kit and followed by western blot analysis with HDAC4 antibody. GAPDH and histone 3 are shown as loading controls for the cytoplasmic and nuclear fractions, respectively.