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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Org Biomol Chem. 2015 Mar 28;13(12):3648–3653. doi: 10.1039/c4ob02502d

Fig. 2.

Fig. 2

A) Schematic procedure of alkene-metabolic labeling and subsequent detection of histones via the oxidative Heck reaction using 3-(biotinylamino)phenylboronic acid 1. B) On blot luminescence imaging and Coomassie Blue staining of histones (5.5 µg) after 6 h incubation with 5 and 10 mM of 4a) sodium pent-4-enoate, 4b) S-propyl pent-4-enethioate and 4c) methyl 4-pentenoate. C) On blot luminescence imaging and Coomassie Blue staining of alkenylated histones (4.0 µg) after 14 h incubation with 30 µM of C646 and 0.41 µM of SAHA followed by treatment with 10 mM of the reporter 4a. D) On blot luminescence detection and Coomassie Blue staining of acetylated histones (3.0 µg) after 20 h incubation with 30 µM of C646 and 0.41 µM of SAHA using the anti-acetyl lysine Antibody.