Increased cytokine release by CD151−/− BMMCs after IgE/Ag stimulation. Cytokine release by BMMCs was measured in culture supernatants by ELISA. BMMCs were unprimed or primed with IgE-DNP, then exposed to 100 ng/ml DNP-HSA for 3 h (A) or 24 h (B) and culture supernatants were tested for levels (pg/ml) of IL-4, IL-13, and TNF-α. (C) Comparison of stimulation effects of IgE, SCF, and PMA on cytokine release by WT and CD151 BMMCs. ELISA data represent the mean ± SEM from four individual assay wells/group over two independent experiments. Filled columns indicate WT; open columns indicate CD151−/−. *p < 0.05.