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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Biochem Pharmacol. 2015 Dec 21;102:130–140. doi: 10.1016/j.bcp.2015.12.010

Fig. 3.

Fig. 3

C646 pre-treatment reduces pro-inflammatory gene expression of TNFα (A), iNOS (B), IL-1β (C), and IL-12b (D) in precision-cut lung slices (PCLS). PCLS were pre-treated with C646 at 1, 5, 10, 15, 20, 25 or 30 μM for 16 hours, after which an inflammatory LPS and IFNγ stimulus (10 ng/mL of each) was given for 4 hours in continued presence of the inhibitors (making the total incubation time with the inhibitors 20 hrs). Subsequently, gene expression was analyzed by RT-q-PCR. For vehicle treatment, PCLS were pre-treated with a proportional dilution of the inhibitor solvent DMF. Data represent the target gene expression normalized to the reference gene. The values shown are means ± SD of 3-6 independent experiments. *** p < 0.001, ** p < 0.01 and * p < 0.05 compared to vehicle.