Fig 5.

MGMT expression was depleted in all cadmium-transformed clones at the protein (A) and mRNA level (B) (**p < 0.001). (C) MGMT was epigenetically silenced in cadmium clones. Cells were treated with an inhibitor of histone acetylation (NaBut; sodium butyrate) or dna methylation (5azaC; 5-aza-2'-deoxycytidine), or a combination of the two, and MGMT mRNA levels were assessed by qRT-PCR. MGMT mRNA levels were upregulated in cadmium clones after treatment with the epigenetic inhibitors, but remained relatively unchanged in control clones.