Fig 1. Trib2 is not required to maintain murine T-ALL cell lines.

Jurkat cells were transduced with normalized viral particles to express shRNAs against Luciferase (shLuc) or Trib2 (shTrib2) along with a GFP reporter. A) The percentage of GFP⁺ cells within the population was monitored for 7 days post-infection. B) At 3 days post-transduction, apoptosis was measured by Annexin V staining (“*”, p = 0.018) and C) Trib2 expression was measured by qPCR 3 days post-transduction in sorted GFP⁺ cells. Errors bars represent the standard deviation of biological replicates. D) T6E cells were transduced with shRNAs against a scrambled sequence (shScrambled) or Trib2 (shTrib2) and GFP as a surrogate marker. GFP⁺ cells were sorted 48 hours after transduction (day 0) and growth was monitored. E) TAL-130 cells were transduced with shRNAs against a scrambled sequence (shScrambled) or Trib2 (shTrib2) and GFP as a surrogate marker. GFP⁺ cells were sorted 48 hours after transduction (day 0) and growth was monitored. F) TAL1 expression was measured in T6E and TAL-130 cells by immunoblot. Jurkat lysates were used as a positive control. G & H) Trib2 expression was measured at days 0 and 15 in T6E (Panel G) or TAL-130 cells (Panel H). Error bars indicate standard deviation. Data are representative of 3 experiments.