Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 17;12(5):e1005651. doi: 10.1371/journal.ppat.1005651

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Chan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 1 — (A) Western blot of myc-tagged Sm.5HTR (Genbank KF444051.1) in HEK293 cells. L, ladder. (B) Schematic of GloSensor assay depicting generation of cAMP by activation of Sm.5HTR (blue) which couples to endogenous G proteins and adenylate cyclase to increase cAMP levels. cAMP binding to the permuted luciferase construct leads to enhanced luminescence in the presence of substrate. (C) Functional expression of Sm.5HTR using this assay. In cells transfected with the biosensor (22F variant) addition of 5-HT (10μM) caused an increase in luminescence in Sm.5HTR transfected (blue symbols), but not control cells (open symbols).