Table 1. Summary statistics for live cell GloSensor cAMP assays on Sm.5HTR.

Z’ factor, signal window and EC₅₀ were calculated at 60 minutes after delivery of 5-HT (10μM).

Adherent Cells	F20		F22
	(-) IBMX	(+) IBMX	(-) IBMX	(+) IBMX
Z’ factor*	0.4	0.7	-0.1	0.7
Signal window**	3.1	3.6	-0.3	12.3
EC50	0.7 μM	0.9 μM	16.1 μM	2.0 μM
Suspension Cells	F20		F22
	(-) IBMX	(+) IBMX	(-) IBMX	(+) IBMX
Z’ factor*	0.9	0.2	0.7	0.9
Signal window**	37.5	0.7	7.4	40.8
EC50	1.4 μM	1.0 μM	4.2 μM	3.8 μM

* Z’ factor calculated for vehicle control and 5-HT (10μM); Z’ = 1 - (3(stdev_max + stdev_min) / (mean_max—mean_min).

** Signal window calculated for vehicle control and 5-HT (10μM), defined as SW = (mean_max—mean_min—3(stdev_max + stdev_min)) / stdev_max.