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a. mRNA expression of the downstream target ID2. B2M was used for normalization. Data are relative to the mean ΔCt of control cells and are expressed as mean±SEM. *p<0.05, **p<0.01, ***p<0.001. b. Western blot analysis of BAR-T and OE33 cells incubated with BMP4 showed upregulated ID2 expression and more phosphorylation of SMAD1/5/8. Cells incubated with Noggin showed downregulated ID2 expression. Actin was used as loading control.
