Table 2. Primer sequences.

Target	Forward primer	Reverse primer	Annealing temperature (°C)
BMP4	TGAGCCTTTCCAGCAAGTTT	CTTCCCCGTCTCAGGTATCA	57.5
SMAD1	CACAAACATGATGGCGCCT	CATAGTAGACAATAGAGCACCAGTGTTT	58
SMAD4	TCCCGGACATTACTGGCCTGTTCA	GCGATCTCCTCCAGAAGGGTCCA	57.5
SMAD5	TTCTGGCTCAATCTGTCAACC	GGAGCCATCTGAGTAAGGAC	57.5
ID2	ACTCGCATCCCACTATTGTC	CGTCCATTCAACTTGTCCTCC	60
CDH1	CGAGAGCTACACGTTCACGG	GGGTGTCGAGGGAAAAATAGG	58
CDH2	TGGGAATCCGACGAATGG	TGCAGATCGGACCGGATACT	58
Vimentin	GGAAGCCGAAAACACCCTG	GAGACGCATTGTCAACATCCT	58

Primer sequences used for Q-RT-PCR with corresponding annealing temperature