Fig 4. Determination of CD4 versus CD8 T cell responses to mutant peptide vaccines.

Mice (n = 2 per group) were vaccinated as in Fig 2. On day 28, splenocytes were harvested from vaccinated mice, incubated overnight in media containing the indicated mutant peptides or media alone, and analyzed by flow cytometry. Cells were gated on viability and lack of MHCII expression (activated murine T cells are MHCII negative). The percentage of IFN-γ-secreting CD8 and CD4 T cells was determined. A. Example of a mixed T cell response (CD8 top and CD4 bottom) to mutant Dync1h1 29mer peptide versus media alone. B. Summary of data for 7 mutant peptides and the positive control peptide long-SIINFEKL. Bars and dots represent mean and individual responses, respectively.