Table 2. Allele frequency calculations in flies yielding usable profiles.
| Locus | T-0 full blood meal | T-0 partial blood meal | T-1 | T-2 | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D | P | D | P | D | P | P | ||||||||||||
| a | b | c | d | b | c | d | c | d | a | b | d | a | c | d | a | d | d | |
| D8S1179 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- |
| D21S11 | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/+ | -/- | +/+ | +/- | +/+ | +/+ | -/- | +/+ | +/- | +/+ | +/+ | +/- |
| D7S820 | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/+ | -/- | +/+ | +/- | +/+ | +/- | +/- | +/+ | +/- | +/+ | +/+ | -/- |
| CSF1PO | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/- | -/- | +/+ | +/- | +/+ | +/- | -/- | +/+ | +/- | +/+ | +/+ | +/- |
| D3S1358 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/- | +/+ | +/+ | +/- | +/+ | +/- | +/+ | +/+ | +/- |
| THO1 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/- | +/+ | +/- | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
| D13S317 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/+ |
| D16S539 | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/+ | -/- | +/+ | +/+ | +/+ | +/- | +/+ | +/+ | +/+ | +/+ | +/+ | +/- |
| D2S1338 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/- | +/+ | +/+ | +/- | +/— | +/- | +/- | +/+ | +/+ |
| D19S433 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/+ | +/- | +/- | +/+ | +/+ | +/+ | +/+ |
| vWA | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/+ | +/- | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/+ | +/+ | +/+ | +/+ |
| TPOX | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/+ | +/+ | +/- | +/- | +/+ | +/- | +/- | +/+ | +/- |
| D18S51 | +/+ | +/+ | +/+ | +/+ | -/+ | +/+ | +/+ | +/- | +/+ | -/- | +/+ | +/- | +/- | +/+ | +/- | +/- | +/+ | +/- |
| D5S818 | +/+ | +/+ | +/+ | +/+ | -/+ | +/+ | +/+ | -/- | +/+ | +/+ | +/+ | +/+ | -/- | +/+ | +/+ | +/+ | +/+ | +/- |
| FGA | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/- | +/+ | +/+ | +/+ | +/- | +/+ | +/+ |
| ΔTotal | 15 | 15 | 15 | 15 | 10 | 15 | 15 | 7 | 15 | 15 | 15 | 15 | 12 | 15 | 15 | 15 | 15 | 13 |
| * RMP, 1 in | 2.4E+21 | 2.0E +21 | 2.4E +21 | 2.4E + 21 | 1.6E + 12 | 6.2E + 20 | 1.3E + 20 | 1.3E +11 | 2.4E +21 | 1.1E +13 | 6.2E +20 | 1.8E +14 | 3.4E +10 | 6.2E +18 | 1.8E +15 | 1.1E +16 | 6.2E +20 | 3.5E +12 |
(D and P) source of human blood meals; (a-d) individual flies with usable STR profiles;
(++) both alleles of heterozygous loci were readable, or in homozygous loci the single peak was above stochastic threshold (ST);
(+/-) only one allele of heterozygous loci was readable or in homozygous loci the RFU value of the allele was below ST;
(-/-) both alleles were not detectable;
(Δ) total number of markers of which at least one allele was readable;
(*) RMP was calculated for each sample based on population specific allele frequencies.