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Molecular weight determined by MALDI-TOF of the PEGylated and unPEGylated-SK mutants were in agreement with their calculated molecular weight. (A) SK1-383N-PEG10 (B) SK1-383C-cys (C) SK1-383N-PEG20 (D) SK1-383C-PEG10 (E) nSK (F) SK1-383C-PEG20.
