Table 1. Genotyping results for individual V. destructor collected in seven locations in the Southeastern USA using the TaqMan® allelic discrimination assay.
Location1 | Sample Name2 | Collected mites3 | HOM L9254 | HOM I925 | HOM M925 | HET L925/I925 | HET L925/M925 | HET M925/I925 | Total Analysed5 |
---|---|---|---|---|---|---|---|---|---|
Dunnellon, FL | Treated_1 | 16 | 0 | 16 | 0 | 0 | 0 | 0 | 16 |
Treated_3 | 8 | 1 | 7 | 0 | 0 | 0 | 0 | 8 | |
Control_1 | 30 | 15 | 3 | 4 | 0 | 2 | 0 | 24 | |
Control_2 | 30 | 6 | 0 | 2 | 0 | 0 | 0 | 8 | |
Micanopy, FL | Treated_2 | 16 | 0 | 12 | 1 | 0 | 0 | 2 | 15 |
Control_3 | 30 | 19 | 1 | 3 | 2 | 1 | 0 | 26 | |
Fort Myers, FL | Treated_1 | 15 | 0 | 6 | 2 | 0 | 0 | 0 | 8 |
Treated_2 | 22 | 1 | 11 | 5 | 0 | 0 | 1 | 18 | |
Control_2 | 30 | 11 | 1 | 9 | 0 | 4 | 0 | 25 | |
Trenton, FL | Treated_1 | 5 | 0 | 3 | 0 | 0 | 0 | 2 | 5 |
Treated_2 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | |
Treated_3 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | |
Control_2 | 19 | 12 | 0 | 4 | 1 | 0 | 0 | 17 | |
Kissimmee, FL | Treated_1 | 14 | 0 | 11 | 0 | 0 | 0 | 0 | 11 |
Control_2 | 30 | 11 | 8 | 3 | 1 | 2 | 0 | 25 | |
Chipley, FL | Treated_1 | 7 | 0 | 4 | 0 | 0 | 0 | 0 | 4 |
Treated_3 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | |
Control_1 | 25 | 4 | 6 | 2 | 3 | 1 | 0 | 16 | |
Sparta, GA | Control_2 | 25 | 13 | 5 | 6 | 1 | 0 | 0 | 25 |
1. Location where sample was collected.
2. Treated: Mites surviving three-day treatment with tau-fluvalinate. Control: mites from the same location but collected from chambers not-treated with tau-fluvalinate.
3. Total number of Varroa collected per sample.
4. Number of mites from each genotype detected in the sample. HOM: Homozygous HET: Heterozygous. L925: wild-type allele, others are mutants.
5. Total number of mites analysed.