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. 2016 May 18;12(5):e1005658. doi: 10.1371/journal.ppat.1005658

Fig 4. GP63 is responsible for the down-modulation of VAMP3 and VAMP8 in L. mexicana-infected macrophages.

Fig 4

BMM were infected with serum-opsonized stationary phase L. mexicana (WT, Δcpb, Δcpb+CPB and Δcpb+GP63) promastigotes for 2 h and 72 h. Total cell extracts were analysed by Western blot (A). Similar results were obtained in three independent experiments. VAMP3 and VAMP8 recruitment to the phagosome was visualized by immunofluorescence microscopy and quantified for 300 phagosomes on triplicate coverslips (B and C). Representative pictures from each condition are shown (D and E). Immunofluorescence assays were performed on 300 phagosomes on triplicate coverslips for three independent experiments. *p<0.0001. Original magnification X63.