Fig 1. WtsE interacts with two maize PP2A B’ subunit proteins in yeast-two hybrid assays, and the deletion of the yeast PP2A B’ gene alleviates WtsE-induced cell death in yeast.

(A) The N-terminal half of WtsE (WtsE-N’, aa 1–964) interacts with two maize PP2A B’ subunit proteins (WIP1 and WIP2). Interaction by yeast-two hybrid is selected on synthetic drop-out (SD) media lacking leucine, tryptophan, and adenine. EV: empty vector control. Pictures taken at 48 h are representative of three biological replicates. (B) Deletion of yeast RTS1 (PP2A B’) allows better growth of yeast cells expressing full-length WtsE. WtsE expression is induced by galactose (2%), and is suppressed by glucose (2%). Pictures, taken at 96 hours after plating of a dilution series, show a representative of three biological replicates.