Fig 3. Transcripts of specific Arabidopsis PP2A B’ regulatory subunit are induced upon Pto DC3000 infection dependent on T3SS.

(A) Protein phylogeny of Arabidopsis and maize PP2A B’ subunit proteins, including WIP1 and WIP2. Accession numbers starting with “AT” are Arabidopsis proteins; accession numbers starting with “NP” or “AFW” are maize proteins. (B) Expression of a subset of Arabidopsis B’ regulatory subunits is induced by Pto DC3000, but not by the type III secretion-deficient mutant Pto hrcC-. Five-week-old Col-0 Arabidopsis leaves were infiltrated with the indicated bacterial strains (10⁸ CFU/ml) or buffer (10 mM MgCl2). Samples were collected at 9 hai and subjected to qRT-PCR. Values shown are mean ± SD of normalized data from three biological replicates with transcript level in buffer treated samples set to 1. ** indicates a significant difference by Student’s t-test at P<0.01.