Table 1. WtsE interacting proteins (WIPs) from yeast-two-hybrid screening.

Name	Y2H positive cDNA clones	Gene accession No.	Gene annotation	Closest homologs	Notes
WtsE-N’ interacting proteins
WIP1	N1-1, N1-3	NM_001153392	Protein phosphatase 2A B’ regulatory subunit	AT3G09880: PP2A B’β (At); AAP68376.1 (Os)	nt 501–1059 of full-length (1569bp) cDNA
WIP2	N1-8, N1-9	NM_001155502	Protein phosphatase 2A B’ regulatory subunit	AT5G25510: PP2A B’ι (At); CAC85920: PP2A B’ι/κ (Os).	nt 90–909 of full-length (1103bp) cDNA
WIP3	N1-2, N1-4, N1-5, N1-6, N1-7	BT060755.1	Leucine-rich repeat receptor-like kinases	AT5G43020 (At); AT5G67200 (At); CAE04495 (Os).	cDNA clones contain the C’-terminal kinase domain.
					Protein sequence similarity with:
					DIPM1—50%
					DIPM2—38%
					DIPM3—49%
					DIPM4—34%
WIP4	N2-9	BT054783	Leucine-rich repeat receptor-like kinases	AT1G10850: LRR-RLK (At); NP_001048614 (Os).	Protein sequence similarity with:
					DIPM1—41%
					DIPM2—36%
					DIPM3—40%
					DIPM4—36%
WIP5	N2-18	BT088209	Leucine-rich repeat receptor- like kinases	AT1G48480: Receptor-like kinase 1 (At); AAN05336 (Os).	Protein sequence similarity with:
					DIPM1—40%
					DIPM2—59%
					DIPM3—36%
					DIPM4—57%
WtsE-C’ interacting proteins
WIP6	C1-1, C1-4, C1-5, C1-6, C1-7	NM_001175852	Ankyrin repeat family protein	AT2G44090 (At); AT3G59910 (At).	Weak homology to AAD41595: Pristionchus pacificus CED-3 protein
WIP7	C1-3, C1-9, C1-10	NM_001154512	Ankyrin domain-containing proteins	AT5G07270: E3 ubiquitin-protein ligase XBAT33 (At); BAC98626: Xa21-binding protein 3-like (Os).
WIP8	C1-8	NM_001152611	Hypothetical protein	AT2G13690: putative PRLI-interacting factor (At); NP_001052356: Similar to PRLI-interacting factor G (Os).

DIPM: DspA/E-interacting protein of Malus ×domestica. DIPM sequences were retrieved from Meng et al., 2006 [41]. WIP3, 4, and 5 protein sequences were aligned with the four DIPMs using BlastP algorithm on NCBI (http://blast.ncbi.nlm.nih.gov/).