Table 2. Partial list of mutations that permit yeast to better tolerate WtsE expression.
| Orf Name | Gene | Gene Function | Size Difference (Ctrl mean-Expt mean) |
|---|---|---|---|
| PP2A-related genes | |||
| YDR435C | PPM1 * | C-terminal protein carboxyl methyltransferase activity | -1.283027 |
| YOR014W | RTS1 | Protein phosphatase type 2A activity | -0.648948 |
| YPL152W | RRD2 | Peptidyl-prolyl cis-trans isomerase activity | -0.555954 |
| YDL134C | PPH21 | Protein phosphatase type 2A activity | -0.553447 |
| Sphingolipid biosynthesis-related genes | |||
| YCR034W | FEN1 * + | Fatty acid elongase activity | -1.228157 |
| YDR497C | ITR1 + | Myo-inositol transmembrane transporter activity | -1.200737 |
| YGR143W | SKN1 + | Glucosidase activity | -0.965214 |
| YDR072C | IPT1 + | Transferase activity, transferring phosphorus-containing groups | -0.763681 |
| YEL042W | GDA1 + | Guanosine-diphosphatase activity | -0.541537 |
| YPL057C | SUR1 | Mannosyltransferase activity | -1.261966 |
| YDR297W | SUR2 | Sphingosine hydroxylase activity | -0.784637 |
| YHL003C | LAG1 | Sphingosine N-acyltransferase activity | -0.636515 |
Ctrl mean: colony size (pixel area) of Gus1-expressing yeast double mutant strain. Expt mean: colony size (pixel area) of WtsE-expressing yeast double mutant strain. Colony size is normalized by dividing each colony size value by the mean colony size across a particular plate from which the colony was derived.
* indicates hits called by both automated and manual scoring. All other hits are from automated scoring only.