Figure 4. DMB attenuates choline enhanced foam cell formation and atherosclerosis.
(A) Representative Oil-Red-O/hematoxylin staining of peritoneal macrophages, Scale bar, 50 μm, and (B) foam cell quantification, from 20 week old male C57BL/6J Apoe−/− mice fed chemically defined chow (0.07% total choline) or 1% choline-supplemented diets from time of weaning (4 weeks). Data are mean ± SE. (C) Representative Oil-Red-O/hematoxylin staining of aortic root sections from 20 week old male C57BL/6J Apoe−/− mouse fed chemically defined chow (0.07% total choline) or choline-supplemented (1.0% total choline) diets in the presence vs. absence of DMB provided in the drinking water, as described under Experimental Procedures. Scale bar, 200 μm. (D) Aortic root lesion area was quantified in 20 week old male C57BL/6J Apoe−/− mice from the indicated diet and DMB treatment groups. Data are mean ± SE. p values shown were calculated by ANOVA.