Table 1.
Expression level of genes involved in pili formation detected in planktonic and biofilm-associated cells of the MAR002 strain, assessed by qRT-PCR.
| Gene expression level |
||||
|---|---|---|---|---|
| Gene code in MAR002 (JRHB01000001/2 and NZ_JRHB01000001/2) | Gene code in ATCC 17978 (CP000521.1) | Putative functions in ATCC 17978 | Exponential planktonic cells | Biofilm-associated cells |
| LH92_03275 LH92_RS03235 | A1S_0690 | FilA | 0,0022 ± 0,0009 | 0,0022 ± 0,0008 |
| LH92_03820 LH92_RS03765 | A1S_3569 | Hypothetical protein | 0,0013 ± 0,0002 | 0,0033 ± 0,0011 |
| LH92_07655 LH92_RS07535 | A1S_1507 | Fimbrial protein | 0,0002 ± 0,00005 | 0,0038 ± 0,0028 |
| LH92_07670 LH92_RS07550 | A1S_1510 | Fimbrial protein | 0,1863 ± 0,0958 | 0,8259 ± 0,1918 |
| LH92_ 08970 LH92_RS08835 | Not found | — | 0,3566 ± 0,0633 | 0,6948 ± 0,0647 |
| LH92_08985 LH92_RS08850 | Not found | — | 0,0198 ± 0,0035 | 0,0816 ± 0,0105 |
| LH92_11070 LH92_RS10905 | A1S_2088 | Hypothetical protein | 0,0016 ± 0,0005 | 0,0054 ± 0,0005 |
| LH92_11085 LH92_RS10920 | A1S_2091 | Putative exported protein | 0,1083 ± 0,0108 | 2,7051 ± 0,5923 |
| LH92_11710 LH92_RS11540 | A1S_2218 | CsuA/B | 0,4429 ± 0,0770 | 0,1485 ± 0,0189 |
| LH92_16105 LH92_RS15875 | A1S_3167 | PilY1 | 0,0979 ± 0,0169 | 0,1410 ± 0,0552 |
| LH92_16175 LH92_RS15945 | A1S_3177 | Fimbrial protein | 0,1158 ± 0,0618 | 0,0697 ± 0,0068 |