Figure 3.

(A) Immunofluorescence analysis of mitochondrial transmembrane disruption after Mtb wild-type and NDMs infection compared to uninfected cells and staurosporine treatment (positive control). Red scattered staining in THP-1 cells indicate undamaged mitochondria; however, cells with altered mitochondrial membrane stains green as the dye accumulates in the cytoplasm and remains in its monomeric form. (B) The percentage of necrosis quantified in 2 hundred cells that was infected with either the Mtb wild-type or NDMs at MOI of 10. **, p < 0.01 and *, p < 0.05, the significance of differences between NDMs and the wild-type.