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. 2015 Nov 25;7(1):23–32. doi: 10.1080/21505594.2015.1102832

Figure 5.

Figure 5.

(A) Necrosis in THP-1 cells that was infected with the Mtb wild-type and overexpressed clones of  lpdC, rplL, glyA1, mihF, glcB, glnA1 and Rv2626c was measured by the LDH activity assay. The MOI used was 10. Results represent means ± standard error of the mean of 3 independent experiments. *, p < 0.05, the significance of differences between Mtb overexpressed clones and the wild-type. (B) Infection and survival assay for Mtb knockout and complemented clone of Rv2626c in vitro. THP-1 cells were infected with Mtb wild-type, Mtb (Rv2626c−) and Mtb (Rv2626c+) clones and CFUs were recorded at 1 h and 5 days post-infection. Results that represent means ± standard error of the mean of 2 independent experiments indicate that Mtb knockout and complemented clones invade and survive in macrophages similarly to the wild-type strain. (C) The Mtb (Rv2626c−) infection of THP-1 cells demonstrates significantly less necrosis when compared to Mtb wild-type and Mtb (Rv2626c+) infection. Cells were infected with an MOI of 10. **, p < 0.01 and *, p < 0.05, the significance of differences between Mtb (Rv2626c−) and the wild-type and complemented clone.