Table 2.

Equations used in time-resolved fluorescence anisotropy measuremnts

Measurement	Formula	Parameters	Eq.
TR-FA decay	$$r(t)=\frac{I_{\text{II}}(t)-G{I}_{\perp }(t)}{I_{\text{II}}(t)+2G{I}_{\perp }(t)}$$	Where t is the time after fluorophore excitation	6
TR-FA fit (for one component)	$$r(t)=r_{\infty }+(r_0-r_{\infty })e^{(-t/{\theta }_{\mathit{rot}})}$$	r∞ – anisotropy after complete depolarization (usually r∞ ≈ 0)	7