Table 3.

Summary of parameters to consider in selection of an assay technology

	FA	TR-FRET	EFC	AlphaLisa/AlphaScreen	SPA
Radiation	No	No	No	No	Yes
Read-out	Fluorescence	Time gated emission	Chemilunescence	Fluorescence	Scintillation
Scalability, max	1536	1536	1536	1536	384
Standard plate readers	Yes	Yes	Yes	Yes	No
Compound interference	High, low with red dyes	Low	Low	Medium	High, low with red beads and plates
Sensitivity	Medium	Medium	High	High	High
Robustness, Z-factor	0.5–0.75	0.7–0.8	>0.7	>0.8	0.5–0.8