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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Anal Bioanal Chem. 2007 May 23;388(8):1643–1651. doi: 10.1007/s00216-007-1301-4

Table 1.

HPLC conditions

Time (min) Solvent A: H2O, 0.1%
formic acid		 Solvent B: ACN, 0.1%
formic acid		 Valve position
0 99% 1% A: Load sample,
  wash
2.2 99% 1% B: Elution
20.0 70% 30% B: Elution
25.0 50% 50% B: Elution
27.0 0% 100% B: Elution
32.0 0% 100% B: Elution
35.0 99% 1% B: Elution

Pump C: Constant flow of 0.1 mL/min ACN, 0.1% formic acid