Table 4.
Mean recovery rates of neuropetides from spiked urine samples from three different individuals after on-line SPE–HPLC tandem MS analysis
| Recovery [%] ± SD | Donor 1 | Donor 2 | Donor 3 | Average |
|---|---|---|---|---|
| Spike 1: 250 ng/mL | ||||
| Gliadinomorphin | 75.3±0.8 | 82.6±4.2 | 81.1±1.6 | 79.7±3.9 |
| Casomorphin | 93.3±2.8 | 91.6±2.4 | 88.5±2.4 | 91.1±2.5 |
| Deltorphin 2 | 94.7±0.3 | 94.3±3.8 | 90.7±3.3 | 93.2±2.2 |
| Deltorphin 1 | 76.3±1.8 | 81.9±3.6 | 76.1±2.9 | 78.1±3.3 |
| Spike 2: 428 ng/mL | ||||
| Gliadinomorphin | 82±2.3 | 82.5±1.9 | 83.7±2.8 | 82.7±0.9 |
| Casomorphin | 98.7±1.7 | 90.2±1 | 86±0.3 | 91.7±6.5 |
| Deltorphin 2 | 96.8±1.5 | 95±0.7 | 88.1±1.5 | 93.3±4.6 |
| Deltorphin 1 | 82±2.5 | 82.8±1.5 | 75.9±0.9 | 80.2±3.8 |
| Spike 3: 643 ng/mL | ||||
| Gliadinomorphin | 85.2±2.2 | 84.7±2.7 | 84.7±0.5 | 84.8±0.3 |
| Casomorphin | 98.1±0.5 | 89.7±1.5 | 84.6±1.5 | 90.8±6.8 |
| Deltorphin 2 | 98.7±0.4 | 94.9±0.2 | 88±0.9 | 93.9±5.4 |
| Deltorphin 1 | 84.3±0.7 | 83.1±2.3 | 74.9±1.7 | 80.8±5.1 |
Mean recovery is given in percent with standard deviation (n=3).