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. 2016 May 18;14(5):e1002461. doi: 10.1371/journal.pbio.1002461

Fig 8. Overexpression of GFP-GEF1 in abi1/abi2/hab1/pp2ca plants partly rescues lateral root growth deficiency of pp2c quadruple mutant.

Fig 8

(A) Quantification of average lateral root (LR) length in wild-type, abi1-1, abi1/abi2/hab1/pp2ca, and pUBQ-GFP-GEF1/abi1abi2hab1pp2ca overexpression plants. Twenty 4-day-old seedlings grown on 1/2 MS medium with similar primary root lengths were transferred onto 1/2 MS medium or 1/2 MS medium supplemented with 0.1 μM IAA, 10 μM ABA, or 0.1 μM IAA plus 10 μM ABA (IAA was used here to accelerate lateral root growth). After the indicated times of growth on hormone-containing or control media, lateral roots were scanned and measured. Note that the bar graphs are from different time points to accurately resolve lateral root lengths for each condition (See Methods). Error bars represent standard deviation (n = 3). (B) Representative image showing lateral root growth of different seedling genotypes grown on 1/2 MS medium supplemented with 0.1 μM IAA. Image was taken 3 d after transferring 4-day-old seedlings to IAA-containing media. An image for similar experiments to those shown in (B) can be found in S7G Fig with 10 uM ABA added. (C) Working model for PP2C-RopGEF-ROP control loop in regulating ABA signal transduction. Arrows indicate activation, and bars indicate repression. Details are described in the text.