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. 2016 May 2;5:e15106. doi: 10.7554/eLife.15106

Figure 4. NMDA receptor-dependent Kalirin-7 expression decreases at puberty.

(a,c,e,g) Representative images, scale, 2.5 μm. Arrows, spines. (a) Phalloidin (Phal), Kalirin-7 (Kal7) and merged images from pre-pub and pub CA1 hippocampus. (b) Mean pixel intensity, *t-test, t(26)=29.2, p<0.0001*, power=1; n=14 neurons (6 mice)/group. (c) Pfn2, Kal7 and merged images from pub WT and α4 KO CA1. (d) Mean pixel intensity, *t-test, t(26)=12.0, p<0.0001*, power=1; n=14 neurons (4 mice)/group. (e) Phal, Kal7 and merged images from pub CA1 hippocampus following in vivo treatment with vehicle or MK801 to increase NMDAR expression (Gao and Tamminga, 1995). (f) Mean pixel intensity, *t-test, t(26)=6.25, p<0.0001*, power=1; n=14 neurons (5 mice)/group. (g) Phal, Kal7 and merged images from post-pub CA1 hippocampus following in vivo treatment with vehicle or memantine (MEM), an NMDAR blocker. (h) Mean pixel intensity, *t-test, t(26)=6.5, p<0.0001*, power=1; n=14 neurons (5 mice)/group. Original uncropped images of Kal7 immunohistochemistry are shown in Figure 4—figure supplement 1. (Figure 4—source data 1) (i) Representative z-stack images, Pub, post-Pub Kal7 KO. Scale, 10 μm. (j) Averaged data, spine density. Proximal: t(32)=0.06, p=0.95, n=17 neurons (6 mice)/group; Distal: t(32)=0, p=1, n=17 neurons (6 mice)/group. (Figure 4—source data 2)

DOI: http://dx.doi.org/10.7554/eLife.15106.023

Figure 4—source data 1. Figure 4b,d,f,h: Measurements of Kalirin-7 (Kal7) luminescence taken from CA1 hippocampal pyramidal cells for Pre-pub and Pub WT (4b), Pub, WT and α4 KO (4d), Pub WT-treated with MK-801 or vehicle (VEH) (4f) and Post-pub WT-treated with memantine or VEH (4h).
DOI: http://dx.doi.org/10.7554/eLife.15106.024
elife-15106-fig4-data1.xlsx (11.5KB, xlsx)
DOI: 10.7554/eLife.15106.024
Figure 4—source data 2. Figure 4j: Spine counts/20 μm on dendrites of CA1 hippocampal pyramidal cells – proximal (left) and distal (right) for pubertal (Pub) and post-pubertal (Post-pub) Kal7 KO mice.
DOI: http://dx.doi.org/10.7554/eLife.15106.025
elife-15106-fig4-data2.xlsx (9.8KB, xlsx)
DOI: 10.7554/eLife.15106.025

Figure 4.

Figure 4—figure supplement 1. Kalirin-7 expression varies across pubertal stage, α4 genotype and level of pubertal NMDAR expression.

Figure 4—figure supplement 1.

Representative images of Kalirin-7 (Kal7) expression in CA1 hippocampus across pubertal stage (a), in pubertal WT and α4 KO hippocampus (b), following pubertal treatment with vehicle or MK-801 to increase NR1 expression (Aoki et al., 2012) (c) or following post-pubertal treatment with vehicle or memantine to block NMDARs (d). Scale, 10 μm. (Averaged data of pixel intensity included in Figure 3).
DOI: http://dx.doi.org/10.7554/eLife.15106.026