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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Bio Protoc. 2015 Dec 20;5(24):e1693. doi: 10.21769/bioprotoc.1693

Figure 1. Isolation, expansion, seeding, and chondrogenic differentiation of BMSCs.

Figure 1

A. A tissue-culture flask (T150) containing isolated BMSCs and expansion medium. B. Adherent, human BMSCs on the surface of a tissue-culture flask demonstrating a characteristic spindle-shaped morphology during expansion (10× magnification). C. Re-suspension of BMSCs in chondrogenic medium within a 50 ml conical tube prior to scaffold seeding. D. Collagen I scaffold placement into the empty well of a 24 well culture plate using forceps. E. Micropipetting of a BMSC-chondrogenic medium suspension onto the central area of a collagen I scaffold. F. Extracellular cartilaginous proteoglycans stained with safranin O following three weeks of chondrogenic differentiation of human BMSCs seeded within a collagen I scaffold. Remnant collagen I scaffold is stained with fast green counterstain [staining protocol described in detail by Bornes et al. (2015); 10× magnification].