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GCGs inhibited AGEs crosslinks formation at the final stage of glycation by G.K. peptide-ribose assay. G.K. peptide (40 mg/mL) was co-incubated with ribose (100 mM) for 9 hrs in the absence or presence of GCGs (at concentrations from 5 to 300 μM). Fluorescence was measured at excitation and emission wavelengths of 340 nm and 420 nm, respectively. Results are shown in means ± SD from three independent tests.
