(A) Representative confocal images of adult animals expressing Punc-129-ELKS-1::Cerulean (tauIs12) in the cholinergic motor neurons. (B) zig-10(0) increased the density of cholinergic synapses. (C) Representative images of adult animals expressing Punc-25-ELKS-1::tdTomato (nuIs249) in the GABA motor neurons. (D) zig-10(0) animals displayed a normal density of GABAergic synapses. (E) Representative images of UNC-63::YFP (kr98) expressed from the endogenous locus (Gendrel et al., 2009). (F) zig-10(0) increased the intensity of cholinergic (UNC-63), but not GABAergic (UNC-49) postsynaptic receptors; data are presented in arbitrary units (a. u.). (G) Representative images of UNC-49::GFP (oxIs22) (Bamber et al., 1999).
Scale bars are 5 µm. The data in B, D, and F are represented as mean ± SEM. ns=not significant, *p<0.05, **p≤0.01, ***p≤0.001, and ****p≤0.0001. Sample size is shown within each bar. See also Figure S4.