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. Author manuscript; available in PMC: 2017 Jan 20.
Published in final edited form as: Neuron. 2016 Jan 20;89(2):325–336. doi: 10.1016/j.neuron.2015.12.024

Figure 4. zig-10 specifically regulates cholinergic synapse density.

Figure 4

(A) Representative confocal images of adult animals expressing Punc-129-ELKS-1::Cerulean (tauIs12) in the cholinergic motor neurons. (B) zig-10(0) increased the density of cholinergic synapses. (C) Representative images of adult animals expressing Punc-25-ELKS-1::tdTomato (nuIs249) in the GABA motor neurons. (D) zig-10(0) animals displayed a normal density of GABAergic synapses. (E) Representative images of UNC-63::YFP (kr98) expressed from the endogenous locus (Gendrel et al., 2009). (F) zig-10(0) increased the intensity of cholinergic (UNC-63), but not GABAergic (UNC-49) postsynaptic receptors; data are presented in arbitrary units (a. u.). (G) Representative images of UNC-49::GFP (oxIs22) (Bamber et al., 1999).

Scale bars are 5 µm. The data in B, D, and F are represented as mean ± SEM. ns=not significant, *p<0.05, **p≤0.01, ***p≤0.001, and ****p≤0.0001. Sample size is shown within each bar. See also Figure S4.