Table 1.
V(D)J Rearrangement of Immunoglobulin Genes of the Isolated CFH-Reactive mAbs
| Ab ID |
VH ID |
VH | DH | JH | VH Mutation Frequency % |
HCDR3 Length |
Isotype | VL ID |
VK | JK | VK Mutation Frequency % |
KCDR3 Length |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ab7970 | H 7970 | 3~30 | 6~13 | 6 | 8.1 | 12 | G3 | K6004 | 4~1 | 1 | 4.6 | 9 |
| Ab7955 | H7955 | 3~30 | 6~13 | 1 | 10.7 | 12 | G3 | K5989 | 4~1 | 1 | 5.4 | 9 |
| Ab7957 | H7957 | 3~30 | 2~21 | 3 | 6.8 | 12 | G3 | K5991 | 4~1 | 1 | 6.3 | 9 |
| Ab7958 | H7958 | 3~30 | 2~21 | 3 | 6.8 | 12 | G3 | K5992 | 4~1 | 1 | 6.3 | 9 |
| Ab7963 | H7963 | 3~30 | 2~21 | 3 | 6.8 | 12 | G3 | K5998 | 4~1 | 1 | 6.3 | 9 |
| Ab7960 | H7960 | 3~30 | 6~25 | 4 | 7.1 | 12 | G3 | K5994 | 4~1 | 1 | 4.1 | 9 |
| Ab7967 | H7967 | 3~30 | 6~25 | 4 | 7.1 | 12 | G3 | K6002 | 4~1 | 1 | 4.1 | 9 |
| Ab7979 | H7979 | 3~30 | 6~25 | 4 | 6 | 12 | G3 | K6015 | 4~1 | 1 | 3.3 | 9 |
| Ab7961 | H7961 | 3~30 | 6~25 | 4 | 6.8 | 12 | G3 | K5995 | 4~1 | 1 | 3 | 9 |
| Ab7965 | H7965 | 3~30 | 6~25 | 4 | 6.8 | 12 | G3 | K6000 | 4~1 | 1 | 3 | 9 |
| Ab7964 | H7964 | 3~30 | 1~26 | 4 | 4.2 | 12 | G3 | K5999 | 4~1 | 1 | 3.5 | 9 |
| Ab7968 | H7968 | 3~30 | 6~13 | 6 | 2.9 | 12 | G3 | K6003 | 4~1 | 1 | 4.3 | 9 |
| Ab7971 | H7971 | 3~30 | 6~13 | 6 | 2.9 | 12 | G3 | K6005 | 4~1 | 1 | 4.3 | 9 |
| Ab7962 | H7962 | 3~48 | 6~6 | 6 | 0.3 | 14 | M | K5996 | 4~1 | 4 | 4.9 | 9 |
| Ab7966 | H7966 | 5~51 | 1~IR1 | 6 | 0 | 17 | M | K6001 | 1~5 | 1 | 0 | 9 |
Antibodies were regarded as clonally related if they were inferred to use the same V and J gene segments, identical CDR3 length and have 70% or greater nucleotide identity in CDR3 region while D-segment identification is subject to substantial uncertainty, so inferred differences in D-segment use were not sufficient to rule out clonal relatedness of two antibodies. Clonally related antibodies are grouped. Based on the analysis, 7 antibodies as highlighted in red color with unique VHDJH and VLJL sequences were selected to be produced as purified antibodies for further characterization.