The SloR-deficient S. mutans GMS584 strain demonstrates compromised survivorship compared to the UA159 wild-type and GMS585 SloR-proficient strains (normalized ZOI ratios = 1.3, 1.0, and 0.84, respectively) when spot inoculated adjacent to streptococcal H2O2 producers., S. mutans was grown overnight to stationary phase and the bacterial concentration was standardized to OD600 = 0.400 ± 0.05 for subsequent spot inoculation., Streptococcus gordonii or S. sanguinis stationary phase cultures grown to OD ∼0.400 ± 0.05 were serially diluted (in triplicate) by a factor of 10 (N = 5 independent experiments). Shown are representative culture competition assays in which either S. gordonii or S. sanguinis was spot inoculated and grown for 16 hours before inoculating the adjacent S. mutans strains (A and C) or all strains were spot inoculated simultaneously (B and D). The S. mutans GMS584 SloR-deficient strain demonstrated the greatest susceptibility to H2O2 challenge.