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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Oral Microbiol. 2016 Feb 2;31(6):526–539. doi: 10.1111/omi.12147

Fig. 3.

Fig. 3

SloR shifts an SRE-containing promoter fragment that precedes the sloABC operon (positive control) but not the sod promoter region or a recA negative control. Shown are the results of EMSA performed on a 6% non-denaturing polyacrylamide gel with [γ-32P] end-labeled sloA-, recA- or sod-specific target sequences and varying concentrations of SloR. Protein-DNA complexes were resolved for a total of 350 volt-hours. Taken together, these findings indicate that the impact of SloR on the sod oxidative stress gene is indirect. The addition of EDTA abrogated the sloABC band shift, consistent with metal ion-dependent SloR binding.