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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Oral Microbiol. 2016 Feb 2;31(6):526–539. doi: 10.1111/omi.12147

Fig. 5.

Fig. 5

SloR shifts a promoter fragment that precedes the S. mutans spxA1 gene. Shown are the results of EMSA performed on a 6% non-denaturing polyacrylamide gel with a 274bp [γ-32P] end-labeled spxA1-specific target sequence and varying concentrations of SloR. SloR-spxA1 complexes were resolved for a total of 350 volt-hours. SloR binds directly to the spxA1-containing DNA fragment when provided at concentrations of 600nM or greater. This binding was abrogated upon addition of the metal ion-chelating agent, EDTA.