Table 2.

List of oligonucleotide primers used in this study.

Assay and primer name	Nucleotide sequence (5’ to 3’)	Annealing temp (°C)	Amplicon size (bp)
cloning
fimA.LR.BamH1.F   fimA.LR.BamH1.R	CGGGATCCCGCAGGATATGGATAA GTGGATTACTGTG CGGGATCCCGGATCTTCTCATCAC GTTCACTGAGTTC	52.5	1664
PCR lig. mut.
fimA-SB-pim-P1	CGCTGGGTGTCATTTTGATTG	53	867
fimA-SB-pim-P2	GGCCGGCCCGGTAAACCAAGCATT GCCTCC
fimA-SB-pim-P3	GGCGCGCCAAAAGTCGGGTGTCTG CG	53	805
fimA-SB-pim-P4	GCCCTTAGTAGAGAGGTATCGT
erm.AscI.F	GGCGCGCCCCGGGCCCAAAATTTG TTTGAT	52.3	860
erm.FseI.R	ATTCTATGAGTCGCTGCCGACTGGC CGGCC
qRT-PCR
SC_sod_qRT_F	ATTGATGCTGAAACGATGACCC	60*	209
SC_sod_qRT_R	AAGAGTTCCCAGAAAAGAGCGTG
SC_tpx_qRT_F	AAATACGGTGACACTTGCTGGTAAG		144
SC_tpx_qRT_R	TCAATAGATGGCACAACGGTAATC
SC_dpr_qRT_F	TGGTTCAGGCTTCCTTTATCTGC		266
SC_dpr_qRT_R	CTTCCTCATCTGTCACATCAAGACC
SC_sloC_qRT_F	CGAAGAAGAGGGAACACCAAATC		186
SC_sloC_qRT_R	CCAGCCTGTCCTTTTTTAGCAAC
Hk11.qPCR.F	GCTGGCTAATAATGTCATCAAGC		88
Hk11.qPCR.F	CTCAACAGTTACTTCAATCTCCTCC
EMSA
MP_sloA_F	ATCGGTGAATCGCACTGTCG	65	364
MP_sloA_R2	GCCATCAATAAAACTTGTCCCTTC
recA.Gel.LN.F	CGGTTATCCAAAAGGGCGTATC	67	212
recA.Gel.LN.R	CCTGTTCTCCTGAATCTGGTTGTG
SC_sod_EMSA_F	GGGGATGATTTCTGTCAAAGCAAG	67	291
SC_sod_EMSA_R	TGTTTTTCAAGAGCCGCATTAGC
SC_dpr_EMSA_F	CGCAATGGAATAGGGTAACCG	66	434
SC_dpr_EMSA_R	TTTGATAAATCCGCTACAGCCTG
SC_tpx_EMSA_F	CGTCTGTCAACTATTCGCAATGC	68	388
SC_tpx_EMSA_R	TTTCTTACCAGCAAGTGTCACCG
JPX_spxA_F	GGGGATGATTTCTGTCAAAGCAAG	63	274
JPC_spxA_R	TGTTTTTCAAGAGCCGCATTAGC

^*The annealing temperature for qRT-PCR primers used in the CFX96 system (Bio-Rad) is 60°C. Restriction enzyme sites appear in boldface type.