Figure 1.

High-throughput screen for identification of inhibitors of human pendrin. A) Schematic of assay showing extracellular addition of SCN⁻ results in pendrin-mediated Cl⁻/SCN⁻ exchange and YFP quenching. B) Representative time course of YFP fluorescence quenching in cells expressing YFP alone (nontransfected, top) and YFP with human pendrin, showing curves for active and inactive compounds (and no test compound, control). C) Chemical structures of pendrin inhibitors identified from screen. D) Structural determinants of activity of class A (left) and C (right) compounds.