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. 2016 Mar 1;30(6):2187–2197. doi: 10.1096/fj.201600223R

Figure 2.

Figure 2.

Characterization of pendrin inhibitors. A) Original fluorescence quenching curves for inhibition of pendrin-mediated Cl−/SCN− (left) and Cl−/I− (right) exchange by PDSinh-A01 (top) and PDSinh-C01 (bottom). B) Concentration-dependent inhibition of pendrin-mediated Cl−/SCN−, Cl−/I−, and Cl−/NO3− exchange by PDSinh-A01 (top) and PDSinh-C01 (bottom). IC50 values for Cl−/SCN− exchange for both compounds were ∼9 μM. PDSinh-A01 and PDSinh-C01 inhibited Cl−/I− and Cl−/NO3− exchange with IC50 of ∼8 µM and ∼5 μM, respectively. C) Kinetics of inhibition of pendrin-mediated Cl−/SCN− exchange in which 10 μM PDSinh-A01 was added for indicated times (left) and summary (right) for PDSinh-A01 and PDSinh-C01. D) Reversibility of PDSinh-A01 and PDSinh-C01. Data shown as means ± sem (n = 4).