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. 2016 Mar 1;30(6):2187–2197. doi: 10.1096/fj.201600223R

Figure 5.

Figure 5.

PDSinh-A01 inhibits Cl−/HCO3− exchange in IL-13-treated primary human bronchial epithelial cell cultures but does not change ASL pH. A) BCECF fluorescence in HBE and CFBE cells in response to extracellular Cl− replacement by gluconate in control (left) and IL-13-treated (right) cells, with PDSinh-A01 (25 μM, 30 min) as indicated. B) Data shown as means ± sem (n = 3–4 cultures) *P < 0.05 comparing without vs. with PDSinh-A01. C) PDSinh-A01 (25 μM, 4 h) does not alter ASL pH, with data shown for HBE and CFBE cells under control and IL-13-treated conditions. Data shown as means ± sem (n = 3–7 cultures). *P < 0.005; **P < 0.05. BCECF ratio imaging indicated initial cytoplasmic pH of ∼7.3 in all cultures studied.