Figure 6.

Inhibition of Cdc42 increases contact guidance–mediated decision making of MDA-MB-231 and HT1080 cells. MDA-MB-231 or HT1080 cells were treated with ML141 (20 µM) or VC. In separate experiments, MDA-MB-231 cells were transfected with control (ctrl) siRNA or siRNA-targeting Cdc42. A, B) Fractions of contact-guided MDA-MB-231 cells (A) and HT1080 cells (B) during the decision-making process. Data show overall fraction of contact-guided cells, and bars show 95% confidence intervals of the fractions. Data were collected and pooled over n ≥ 3 independent experiments. The number of cells assayed for each condition is indicated on the plot. Comparisons between fractions of treated and control cells were analyzed using the 2-population proportion z test. C) Knockdown of Cdc42 in MDA-MB-231 cells was confirmed via Western blot, with actin as loading control. n.s., not significant. *P < 0.05.