Table 1.
Abundance of Aeromonas in chironomid egg masses collected from the Tivon WSP.
| Samplinga | Aeromonas (cfu/egg mass) culturable method | No. of Aeromonas/egg mass real-time PCR method |
|---|---|---|
| 1 (0) | 2.17 × 103 | 9.35 × l04 |
| 2 (12) | 2.47 × l03 | 1.30 × l04 |
| 3 (25) | 5.59 × l03 | 1.02 × l04 |
| 4 (40) | 3.61 × l03 | 1.62 × l04 |
| 5 (65) | ND | 1.71 × l04 |
| 8 (112) | ND | 1.06 × l04 |
| 9 (118) | 2.78 × l02 | 1.10 × l04 |
| 10 (122) | 2.92 × l03 | 2.20 × l04 |
| 11 (127) | 3.97 × l03 | 1.41 × l04 |
| 12 (133) | ND | 8.58 × l03 |
| 13 (140) | 4.38 × l03 | 1.97 × l04 |
| 15 (154) | 2.87 × l03 | 5.31 × l03 |
| 16 (160) | 2.33 × l02 | 5.40 × l03 |
| 17 (167) | 4.51 × l03 | 1.88 × 104 |
Chironomid egg masses were sampled between April and September, 2009. Numbers in the table are the average of three egg masses, three repeats for each (data from Shaked, 2011). Comparison of Aeromonas cfu/egg mass and estimated Aeromonas numbers were estimated by real-time PCR technology using the number of copies of the 16S–23S rRNA intergenic region, according to Kong et al. (1999). aNumbers in parentheses indicate day of sampling. Date of the first sampling was April 1, 2009. ND, Not detectable.