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. 2016 May 19;7:763. doi: 10.3389/fmicb.2016.00763

Figure 4.

Figure 4

Expression of pla2 in non-virulent Y. pestis Kimberley53pCD1−pPCP1− and its derivatives #83 and #66-6. The expression profile of pla2 was determined in the non-virulent Y. pestis Kimberley53pCD1−pPCP1− (parental strain) and its ciprofloxacin reduced-susceptibility derivatives #83 and #66-6. Cultures were prepared from bacteria grown overnight on BIN, suspended in MHB and incubated for 2 h followed by 2 h exposure to the required ciprofloxacin concentrations in a 0.5-ml culture volume in 24-well plates. RNA samples were purified and used for qRT-PCR analysis using 16S rRNA as a reference for FC determination as described in the Materials and Methods. The MIC value for each derivative was also determined by a standard microdilution test performed using the same cultures that were used for the molecular AST. The colored arrows indicate the microdilution-measured MIC values of the bacterial derivatives. The black dotted line represents basal expression (FC = 1) in the growth control sample, and the green dashed line represents the threshold value assigned to pla2 (FC = 3.2). The figure is representative of duplicate analyses.