. 2016 Feb 3;23:9559–9573. doi: 10.1007/s11356-016-6093-7

Table 1.

Data about clutches and eggs that were analysed for pesticide residues and results of GC/MS-MS and LC/MS-MS analyses (mg/kg)

Clutch code	Clutch characteristics				Egg characteristics				Residue analyses (mg/kg)
Clutch code	Fate	Number of eggs laid	% Unhatched eggs^a	% Fertile eggs	Egg status	Stage of development (days)	Difomity^b	Eggshell thickness (mm)	GC/MS-MS screening	LC/MS-MS screening
2011-80-864-1	Failure	6	100	42	Dead embryo	3	–	0.236	ND	ND
					Indet	–	–		ND	ND
					Infertile	–	–	0.212
					Infertile	–	–	0.223
2011-45-24-2	Hatching	14	86	57	Indet	–	–	0.214	ND	ND
					Indet	–	–	0.254	ND	Thiamethoxam(+clothianidin): D = 0.013
					Indet	–	–	0.237
					Indet	–	–	0.241
					Indet	–	–	0.226
					Indet	–	–	0.248	ND	ND
					Indet	–	–	0.251
					Indet	–	–	0.244
2011-45-583-2	Hatching	15	67	80	Dead embryo	15	Yes	0.226	ND	ND
					Indet	–	–	0.246	ND	ND
					Indet	–	–	0.27
					Dead embryo	6	–	0.246
					Dead embryo	10	–	0.22
					Dead embryo	11	–	0.418	Heptachlor(+epoxyde) D < 0.01	ND
					Indet	–	–	0.206
					Indet	–	–	0.283
2011-77J-1153-1	Hatching	10	60	85	Dead embryo	2	–	0.236	ND	ND
					Dead embryo	8	–	0.214	ND	ND
					Indet	–	–	0.212
					Dead embryo	2	–	0.228
					Indet	–	–	0.214	ND	ND
					Indet	–	–	0.237	ND	ND
2011-77P-723-1	Hatching	13	31	96	Dead embryo	22	No	0.198	ND	Bromoxynil D < 0.01
					Indet	–	–	0.218	Heptachlor(+epoxyde) D = 0.11	ND
					Dead embryo	23	No	0.204	Heptachlor(+epoxyde) D = 0.11
					Dead embryo	23	No	0.204	Prochloraz(+TCP) D = 0.041
2011-51-104-3	Hatching	11	27	100	Dead embryo	23	No	0.223	ND	ND
2011-51-104-3	Hatching	11	27	100	Dead embryo	23	No	0.244	ND	ND
2010-76-359-2	Hatching	11	55	91	Dead embryo^c	18	Yes	0.186	ND	ND
2010-80-411-2	Hatching	14	93	100	Dead embryo	23	Yes	.	ND	ND
2011-77J-1158-1	Hatching	11	9	100	Dead embryo	22	Yes	0.239	Prochloraz(+TCP) D = 0.024	ND
2011-14/61-177-1	Hatching	14	7	93	Infertile	–	–	0.175	ND	ND
2011-41-1101-1	Failure	8	–	–	Dead embryo	2	–	0.127	ND	ND
					Dead embryo	2	–	0.237	DDT(Σ isomers) D = 0.046	ND
					Dead embryo	2	–	0.225	DDT(Σ isomers) D = 0.046	ND
2011-14/61-181-1	Hatching	20	5	100	Dead embryo	9	–	0.239	ND	ND
2011-76-952-1	Hatching	14	21	89	Indet	–	–	0.205	ND	ND
					Indet	–	–	0.25	Fipronil(+sulfone) D = 0.0085	ND
					Indet	–	–	0.228	HCH(α + β + δ) D = 0.015	ND
2011-27-1040-1	Hatching	15	20	87	Infertile	–	–	0.205	Cyproconazole D = 0.021	ND
2011-28-799-2	Failure	11	100	–	Indet	–	–	0.215	ND	ND
					Indet	–	–	0.277
					Indet	–	–	0.205
					Indet	–	–	0.26	ND	ND
					Indet	–	–	0.315
					Indet	–	–	0.226
2011-45-586-1	Hatching	18	28	86	Indet	–	–	0.239	ND	ND
					Indet	–	–	0.224	ND	ND
					Indet	–	–	0.239	Cyhalothrin(lambda) D < 0.01	ND
					Indet	–	–	0.258	Fenpropidin D = 0.34
									Heptachlor(+epoxyde) D < 0.01
									Tebuconazole D < 0.01
2011-45-587-1	Hatching	20	30	95	Dead embryo	11	–	0.285	Diphenylamine D = 0.01	ND
					Dead embryo	3	–	0.229
					Dead embryo	12	–	0.251
					Dead embryo	19	No	0.264	Fenpropidin D = 0.036	ND
					Indet	–	–	0.215
					Indet	–	–	0.25
2010-78-760-1	Hatching	19	32	95	Dead embryo	12	–	0.245	ND	ND
					Dead embryo	12	–	0.273
					Dead embryo	12	–	.
					Dead embryo	12	–	.
2011-77J-1200-1	Hatching	18	11	94	Indet	–	–	0.223	ND	ND
2011-77J-1200-1	Hatching	18	11	94	Indet	–	–	0.225	ND	ND
2011-80-871-2	Hatching	13	62	88	Dead embryo	5	–	0.229	Fipronil(+sulfone) D = 0.0083	Thiamethoxam(+clothianidin) D < 0.01
					Dead embryo	5	–	0.238
					Indet	–	–	0.214
					Indet	–	–	0.244	ND	Thiamethoxam(+clothianidin) D = 0.037 of which clothianidin D = 0.032
					Dead embryo	16	No	0.237	PCB153 D < 0.01	–
					Dead embryo	11	–	0.243	PCB153 D < 0.01	–
2011-14/61-153-3	Hatching	10	60	85	Infertile	–	–	0.33	ND	ND
					Dead embryo	6	–	0.293
					Dead embryo	19	No	0.275
					Indet	–	–	0.351	ND	ND
					Dead embryo	19	No	0.239	ND	ND
2011-78-51-2	Hatching	11	36	91	Infertile	–	–	0.241	ND	ND
2011-78-51-2	Hatching	11	36	91	Dead embryo	23	Yes	0.211	ND	ND
2011-59-848-2	Hatching	4	25	100	Dead embryo	9	–	0.23	ND	ND
2011-51-145-2	Hatching	10	30	85	Infertile	–	–	0.243	Diphenylamine D < 0.01	ND
2011-51-145-2	Hatching	10	30	85	Dead embryo	18	No	0.219	Diphenylamine D < 0.01	ND
2010-51-202-1	Failure	16	–	16	Infertile	–	–	0.253	ND	ND
					Dead embryo	4	–	0.232
					Dead embryo	3	–	0.265
					Infertile	–	–	0.235	ND	ND
					Infertile	–	–	0.255
					Infertile	–	–	0.224
2011-80-924-1	Failure	15	–	93	Dead embryo	8	–	0.259	ND	ND
					Dead embryo	8	–	0.267
					Dead embryo	8	–	0.258
2011-77P-719-1	Hatching	16	25	91	Dead embryo	16	No	0.254	ND	ND
					Indet	–	–	0.231	ND	ND
					Indet	–	–	0.234	ND	ND
2010-80-442-2	Failure	8	–	100	Dead embryo	23	No	.	ND	ND
2011-41-1101-2	Failure	9	–	78	Infertile	–	–	0.209	DDT(Σ isomers) D < 0.01	ND
2011-41-1101-2	Failure	9	–	78	Dead embryo	20	No	0.171	DDT(Σ isomers) D < 0.01	ND
2011-41-1073-1	Hatching	12	33.3	100	Dead embryo	22	Yes	0.223	DDT(Σ isomers) D < 0.01	ND
2011-41-1075-1	Failure	7	–	–	Dead embryo	2	–	0.26	DDT(Σ isomers) D = 0.027	ND
					Indet	–	–	0.246
					Dead embryo	2	–	0.25
2011-41-1107-1	Failure	³ 4	–	–	Dead embryo	10	–	0.18	DDT(Σ isomers) D = 0.03	ND
2011-41-1107-1	Failure	³ 4	–	–	Dead embryo	10	–	0.182	DDT(Σ isomers) D = 0.03	ND
2011-45-579-1	Hatching	17	11.76	94	Indet	–	–	0.204	Cyproconazole D = 0.015	ND
2011-45-579-1	Hatching	17	11.76	94	Indet	–	–	0.226	Cyproconazole D = 0.015	ND
2011-41-1066-1	Failure	22	–	100	Dead embryo	15	No		ND	ND
2011-45-658-1	Failure	15	–	100	Dead embryo	20	No	0.238	DDT(Σ isomers) D = 0.016	ND
2011-59-841-1	Failure	15	–	67	Dead embryo	2	–	0.218	Fipronil(+sulfone) D = 0.0068	Thiamethoxam(+clothianidin) D = 0.067 of which clothianidin D = 0.057
					Dead embryo	2	–	0.218	PCB153 D < 0.01
					Dead embryo	2	–	0.234	PCB180 D < 0.01
					Infertile	–	–	0.212	PCB180 D < 0.01
2011-59-803-1	Failure	12	–	83	Dead embryo	4	–	0.25	Prochloraz(+TCP) D < 0.01	ND
					Dead embryo	5	–	0.239
					Dead embryo	4	–	0.253
2011-59-839-2	Hatching	8	12.5	94	Indet	–	–	0.252	ND	ND
2011-80-906-1	Failure	15	–	93	Indet	–	–	0.25	Prochloraz(+TCP) D < 0.01	ND
					Indet	–	–	0.245	Prochloraz(+TCP) D < 0.01	ND
					Dead embryo	21	No	0.21	Prochloraz(+TCP) D < 0.01	ND
2011-80-886-1	Failure	16	–	97	Indet	–	–	0.215	Diflufenican D = 0.016	ND
2011-80-886-1	Failure	16	–	97	Dead embryo	22	No	0.216	Diflufenican D = 0.016	ND
2011-80-917-1	Failure	14	–	100	Dead embryo	21	No	0.234	PCB153 D < 0.01	ND
2011-14-61-168-2	Failure	14	–	96	Dead embryo	20	No	0.242	ND	ND
2011-14-61-168-2	Failure	14	–	96	Indet	–	–	0.22	ND	ND
2011-14-61-175-1	Failure	8	–	–	Dead embryo	10	–	0.236	ND	ND
2011-51-111-1	Failure	14	–	7	Infertile	–	–	0.231	Fenpropodin D = 0.032	ND
2011-51-111-1	Failure	14	–	7	Dead embryo	2	–	0.22	Fenpropodin D = 0.032	ND
2011-51-204-1	Failure	10	–	80	Dead embryo	18	No	0.225	ND	ND
					Dead embryo	18	No	0.221
					Dead embryo	18	No	0.24
2011-51-222-2	Failure	32	–	–	Dead embryo	12	–	0.182	ND	ND
2011-51-222-2	Failure	32	–	–	Infertile	–	–	0.165	ND	ND
2011-77J-1148-1	Hatching	18	11.1	94	Indet	–	–	0.243	ND	ND
2011-77J-1148-1	Hatching	18	11.1	94	Indet	–	–	0.268	ND	ND
2011-77P-1164-1	Hatching	14	21.4	93	Dead embryo	10	–	0.209	ND	ND
2011-77P-1164-1	Hatching	14	21.4	93	Dead embryo	22	Yes	0.238	ND	ND
2011-76-359-1	Failure	13	–	31	Dead embryo	2	–	0.243	ND	ND
					Infertile	–	–	0.23
					Dead embryo	2	–	0.251
2011-27-38-1	Failure	12	–	67	Dead embryo	21	Yes	0.221	ND	ND
2011-76-999-1	Failure	31	–	–	Dead embryo	2	–	0.24	ND	ND
2011-76-958-2	Failure	8	–	63	Dead embryo	20	No	0.238	Difenoconazole D = 0.013	ND
2011-76-958-2	Failure	8	–	63	Dead embryo	20	No	0.238	Diphenylamine D = 0.019	ND

ND no active substances (ASs) detected (LoD approximatively half of LoQ—0.01 mg/kg for most ASs, see Online Resource 1)), – not relevant data

^aCalculated on clutches incubated ≥24 days

^bRecorded for embryo ≥15 days of development

^cOnly the embryo was available for residue analysis