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. 2016 May 19;6:26344. doi: 10.1038/srep26344

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Correlative (A) optical microscopy, (B) 2PEF microscopy, (C) SHG microscopy, (D) AFM topography, nanoIR mapping at (E) 1724 cm⁻¹ and (F) 1660 cm⁻¹. Arrows in the 2PEF microscopy image (B) show hot fluorescent areas in the cluster and are correlated with the nanoIR mapping at 1724 cm⁻¹, corresponding to the gel-carbonyl band.