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. 2016 Jan 20;44(9):4147–4162. doi: 10.1093/nar/gkw008

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Loss of Set2 compromised heterochromatic silencing without affecting heterochromatin structure. (A, B and D) ChIP analyses of H3K36me2 and H3K36me3 (A), Set2-FLAG (B), H3K9me2 (D) and Rpb1 (E) at heterochromatic dh repeats were performed using the indicated strains. Error bars show the standard deviation of three independent experiments. (C) Quantitative RT-PCR (qRT-PCR) analysis of the forward transcript from dh repeats was performed using the indicated strains. Error bars show the standard deviation of three independent experiments.