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. 2016 Feb 11;44(9):4189–4199. doi: 10.1093/nar/gkw087

Figure 5.

Figure 5.

Measurement of D-I03 (A) and D-G23 (B) binding to RAD52. Compound D-I03 at concentrations of 3.125, 6.25, 12.5, 25 and 50 μM or compound D-G23 at concentrations of 1.56, 3.125, 6.25, 12.5 and 25 were injected to the chip with immobilized RAD52. Colored lines indicate experimental data, whereas black lines indicate fitting to the simple 1:1 binding model using the ProteOn Manager Software version 3.0 (Bio-Rad). For D-G23 binding to RAD52, kinetic values are as follows: ka = 1.15 (± 0.44) × 104 M−1s−1; kd = 3.62 (± 0.7) × 10−1 s−1; Kd = 34.0 ± 8.9 μM. (C) For D-I03, the data did not fit to any available binding models and the Kd was determined using equilibrium analysis by plotting the response at equilibrium (Req) versus concentration of compound. Experiments were repeated at least three times; error bars indicate S.D.