Figure 6.

TWINKLE catalyzes homologous branch migration reaction. (A) Gel image shows the time course of HJ resolution of a substrate with one ss-DNA 5′-tail with and without TWINKLE in the presence of MgUTP. (B) Gel image shows control reactions where no HJ resolution was observed in the absence of UTP and absence of TWINKLE. (C) Gel image shows HJ resolution of substrate with two ssDNA 5′-tails in opposite arms. (D) The gels in (A and C) are quantified to obtain the rate of HJ resolution of a substrate with one ss-DNA tail (0.05 ± 0.002 strand/min) and two ssDNA tails (0.02 ± 0.001 strand/min).