Table 2. Differential binding of SAUGI to several UDGs.
| SAUGI to UDGs (1:1) | ka (1/Ms) | kd (1/s) | KD (nM) | Reference |
|---|---|---|---|---|
| SAUGI to SAUDG | 2472386 ± 16363 | 0.00296 ± 0.00010 | 1.198 ± 0.029 | (16) |
| SAUGI to human UDGa | 1846576 ± 130186 | 0.00361 ± 0.00084 | 1.959 ± 0.476 | This study |
| SAUGI to HSVUDG | 3400215 ± 153579 | 0.00173 ± 0.00008 | 0.508 ± 0.038 | This study |
| SAUGI to EBVUDG | 2535637 ± 343747 | 0.00120 ± 0.00019 | 0.475 ± 0.086 | This study |
| SAUGI E24AQ52R to human UDG | 1600443 ± 249179 | 0.00529 ± 0.00085 | 3.304 ± 0.102 | This study |
| SAUGI E24AQ52R to HSVUDG | 6917482 ± 897402 | 0.00111 ± 0.00007 | 0.163 ± 0.033 | This study |
| SAUGI to TBUDG (two-state) | ka1 and ka2 (1/Ms) | kd1 and kd2 (1/s) | KD (nM) | Reference |
| SAUGI to TBUDG | ka1: 4320235 ± 1244152 | kd1: 1.39320 ± 0.21470 | 198 ± 39 | This study |
| ka2: 20.00274 ± 0.00048 | kd2: 0.00400 ± 0.00056 |
aThe binding affinity of SAUGI to human UDG was measured in our previous report (KD = 2.5 nM, (16)). The same assay was also repeated here.