Table 2. Parametric values from mechanistic analysis of binary DB270/DNA/PU.1 interactions. Pairwise interactions between the three components were measured by FP changes (in terms of steady-state anisotropy, <r>) using DB270 (10 nM) or internally Cy3- and Cy5-labeled DNA (1 and 3 nM, respectively) harboring the high-affinity [5′]AGC sequence as probes. DNA sequences are given in Table 1. The nature of the models and notation of the subscripts are as described in the text (c.f. Schemes 1–3) and detailed in Supplemental Methods. All experiments summarized here are performed in 10 mM Tris–HCl, pH 7.4 and 150 mM NaCl. Values are means ± S.E. of N replicate experiments (in parenthesis) in which the parameter was directly estimated.